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2.2 Biochemical analyses Glucose levels were measured using a glucose oxidation method (Architect c8000 instrument, Abbott Diagnostics, Abbott Park, IL)

Alcohol prep pads: Disinfect the vial stoppers before each puncture and let the alcohol dry fully

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Consider using multiple paired methods to provide robust evidence of barrier effects for example, combining TEER measurements with permeability assays and tight junction protein test in cell culture studies, or combining lactulose/mannitol testing with circulating marker measurements in human studies

Thus, while direct causation by semaglutide is not conclusively established, the phenomenon warrants further investigation and discussion with healthcare providers